FIG 3.

The myo5Δ mutant accumulates high levels of membrane-permeant and -nonpermeant dyes as determined by microscopy. (A to D) Cells were incubated with the indicated fluorescent dyes and observed with a microscope. (A) ER-Tracker Red. (B) CellTracker Green CMFDA. (C) MitoTracker Red CMXRos. (D) Propidium iodide (PI). Fluorescent and DIC images were photographed. The staining patterns of each dye were similar between the wild type (WT) and the myo5Δ mutant, but the fluorescent intensity was greater in the myo5Δ mutant than in the wild type. Bars = 5 μm.