FIG 4.

Myosin-I mutants of different fungal species accumulate high levels of membrane-permeant or -nonpermeant dyes. Cells of indicated strains were incubated with each membrane-permeant or -nonpermeant dye, and accumulation of each dye was assessed by flow cytometry. The experiments were repeated three times, and the error bars represent the standard deviations. Ca, C. albicans; Sp, S. pombe. Values that are significantly different are indicated by bars and asterisks as follows: **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001. Values that are not significantly different (P > 0.05) are indicated (ns).