Figure 6.

α‐SNAP_Rhg1LC protein is expressed despite RAC presence. (a) Schematic showing PIPE‐mediated removal of RAC from the native α‐SNAP‐RAC construct, pSM101. (b) Immunoblots of independent “Forrest” or Wm82 transgenic root lysates using previously described antibodies for α‐SNAP_Rhg1LC or WT α‐SNAP proteins. “+” denotes α‐SNAP‐RAC transformation, “−” indicates transformation with α‐SNAP_Rhg1LC (RAC removed), and EV is transformed with empty vector. Ponceau S staining serves as a loading control. (c) Agarose gel showing RT‐PCR amplification of mature α‐SNAP_Rhg1LC transcript isoforms from roots of Wm 82 or “Forrest” transformed with α‐SNAP‐RAC (+), or a native α‐SNAP_Rhg1LC cassette with RAC removed (−), or an empty vector control. WT refers to primers specific for WT α‐SNAP transcripts, LC detects full‐length α‐SNAP_Rhg1LC transcripts, while “Iso” amplifies a previously described α‐SNAP_Rhg1LC alternative transcript isoform that splices out 36 bp (Cook et al., 2014). "...": same WT‐LC‐Iso pattern