Figure 2.

JQ1 induces autophagy in BC cells. A. Cells were transiently transfected with GFP‐RFP‐LC3 plasmid. After 24 h, cells were treated with JQ1 (0.8 μmol/L) for additional 24 h. Autophagy‐like red dots and yellow dots were observed by a fluorescence microscopy and were statistically analyzed. B. Autophagy‐like ultrastructures were observed under the TEM after 24 h treatment of JQ1 (0.8 μmol/L). Rectangle indicates the 4‐fold magnified image. Black arrows point to autophagy‐like ultrastructures