Skip to main content
. 2019 Jun 28;8(10):4792–4805. doi: 10.1002/cam4.2385

Figure 4.

Figure 4

Autophagy induced by JQ1 contributes to proliferation inhibition. Cells were treated with JQ1 (0.8 μmol/L) with/without the presence of vehicle, 3‐MA (5 mmol/L) or BAFA1 (10 nmol/L) or NH4Cl (10 mmol/L) for 48 h, then cell proliferation was determined by MTT assay (A) and cell counting assay (B). C, D, E. Cells were transiently transfected with siATG5 or siControl. After 24 h, cells were treated with JQ1 (0.8 μmol/L) for additional 24 h. The expression of p‐ATG5 and LC‐3B was checked by western blotting analysis (C), cell proliferation was evaluated by MTT assay (D) and cell counting assay (E). *P < 0.05 vs control, # P < 0.05 vs JQ1 in the vehicle group. Data represent the results of three independent experiments