Table 1.
Antibodies used for western blotting analysis, IHC, and endogenous immunoprecipitation assays
| Antibody | Source | Clone, Cat.# | Description | Application, dilution |
|---|---|---|---|---|
| p62 | Cell Signaling Technology | 88588 | Mouse monoclonal |
WB, 1:1000 IHC, 1:200 |
| LC3‐B | Cell Signaling Technology | 3868 | Rabbit monoclonal |
WB, 1:1000 IHC, 1:200 |
| GAPDH | Santa Cruz | sc‐0411 | Mouse monoclonal | WB, 1:10000 |
| p‐LKB1 (Ser428) | Cell Signaling Technology | 3482 | Rabbit monoclonal | WB, 1:1000 |
| p‐LKB1 (Ser428) | abcam | ab138386 | Rabbit polyclonal | IHC, 1:300 |
| LKB1 | Cell Signaling Technology | 3050 | Mouse monoclonal |
WB, 1:1000 IP, 1:100 |
| p‐AMPKα (Thr172) | Cell Signaling Technology | 50081 | Rabbit monoclonal |
WB, 1:1000 IHC, 1:200 |
| AMPKα | Cell Signaling Technology | 5832 | Rabbit monoclonal |
WB, 1:1000 IP, 1:100 |
| p‐ACC (Ser79) | Cell Signaling Technology | 11818 | Rabbit monoclonal | WB, 1:1000 |
| ACC | Cell Signaling Technology | 3676 | Rabbit monoclonal | WB, 1:1000 |
| p‐mTOR (Ser2448) | Cell Signaling Technology | 5536 | Rabbit monoclonal | WB, 1:1000 |
| p‐mTOR (Ser2448) | Cell Signaling Technology | 2976 | Rabbit monoclonal | IHC, 1:200 |
| mTOR | Cell Signaling Technology | 2983 | rabbit monoclonal | WB, 1:1000 |
| p‐ULK1 (Ser555) | Cell Signaling Technology | 5869 | Rabbit monoclonal |
WB, 1:1000 IHC, 1:100 |
| ULK1 | Cell Signaling Technology | 6439 | Rabbit monoclonal | WB, 1:1000 |