Figure 5.

Melatonin inhibits tumor growth and promotes autophagy in vivo. (A) Tumor size was reduced by melatonin treatment. PBS, 40 mg/kg melatonin or 80 mg/kg melatonin was intraperitoneally injected into nude mice once a day for 3 weeks. The horizontal bars indicate the average tumor size. (B) Tumor sections from each group were analyzed for HAS3, GFAP, TUBB3, cytochrome C, and LC3 via IHC staining. (C) Each group of tumor lysates was assessed for HAS3, TUBB3, GFAP, LC3, and GAPDH. The results were normalized to GAPDH. (D) Autophagosomes were detected by TEM in tumor tissue from each group. Magnification, 12 K; scale bar, 1 μm. The data are presented as the mean ± SD; *P < 0.05