Figure 4.

PolyP binding induces conformational changes in the MTBR when the PRR is present. Histograms from smFRET measurements probing the MTBR and PRR in the absence (dark gray and dashed line; left axis) or presence (light gray and solid line; right axis) of 20 μM polyP₃₀₀. Histograms for probes of the MTBR (C244–C372) in 2N4R (a), P2-4R (b), 4R (c), 2N3R (d), P2-3R (e), and 3R (f). Histograms for probes of the PRR (C149–C244) for 2N4R (g), 2N3R (h), and P1P2 (i). Tau schematics above each histogram represent the construct and labeling positions. At least three separate measurements of each condition/construct were made. The histograms shown are representative. The statistical analysis of repeat measurements is given in Table S3. To see this figure in color, go online.