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Cas9 mediates GBS disease progression and vaginal colonization, in vivo. Recovered bacterial CFU (WT COH1 or COH1Δcas9 mutant) in the (A) brain, (B) lungs, and (C) blood of CD-1 outbred mice at 72 h post-intravenous infection. Mann–Whitney U test ∗∗∗p < 0.001. (D) Bacterial load of COH1 and COH1Δcas9 mutant in the vaginal tracts of C57BL/6 mice (n = 10/group) over time. Two-way repeated measures ANOVA with Sidak’s multiple comparisons, ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.
