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. 2019 Aug 21;10:879. doi: 10.3389/fneur.2019.00879

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Copyright © 2019 Wong, Braidy, Pickford, Sachdev and Poljak.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ISTD raw abundances across the three extraction methods in (A) positive ion mode and (B) negative ion mode. The Alshehry method was as effective as the Folch and Matyash method in extracting most lipid classes and was more effective in extraction of more polar lipids (LPC, LPE and PI). Error bars represent SE (*p < 0.05 relative to single phase method). CE, cholesterol ester; D5DG, deuterated diacylglycerol; DG, diacylglycerol; LPC, lysophosphatidylcholine; LPE, lysophosphatidylethanolamine; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol, PI, phosphatidylinositol, PS, phosphatidylserine, SM, sphingomyelin; TG, triacylglycerol.