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. 2019 Jul 16;117(4):751–766. doi: 10.1016/j.bpj.2019.07.011

Figure 4.

Figure 4

Experimental voltage-dependent profile structures for the KvAP-POPC membrane. (A) Left side shows the nSLD(z) profiles for the KvAP-POPC membrane of specimen S2 (black hyperpolarized, gray depolarized) at a solvent contrast of 90% D2O/10% H2O. The profile for the membrane is at negative contrast relative to 90% D2O. (A) Right side shows the difference ΔnSLD(z) profile (hyperpolarized minus depolarized) at the same contrast of 90% D2O/10% H2O. (B) Left side shows the nSLD(z) profiles for the same specimen S2 at a contrast of 60% D2O/40% H2O. The profile for the membrane is also at negative contrast relative to 60% D2O. (B) Right side shows the difference ΔnSLD(z) profile (hyperpolarized minus depolarized) at the same contrast of 60% D2O/40% H2O. (C) Right side shows the ΔnSLD(z) profile (hyperpolarized minus depolarized) for the organic SAM on the inorganic Si-Ge-Si multilayer substrate’s surface, but otherwise lacking the tethered KvAP-POPC membrane, for specimen S1 at a solvent contrast of 90% D2O/10% H2O. The horizontal dashed lines above and below the abscissa were used to estimate the propagation of errors into the difference ΔnSLD(z) profiles and hence the nSLD(z) profiles contributing to the difference. The nSLD(z) profiles are in units of 10−6/Å2.