Figure 2.

T cell population and phenotypic changes of WT and CB1^−/−CB2 ^−/− EAE mice treated with vehicle or THC+CBD. As shown in Figure 1, EAE was induced in naïve mice then mice were treated with Veh or THC+CBD when symptoms appeared. Inguinal lymph nodes and brain MNCs were collected at the peak of disease (Day 15 post immunization). (A) IL-17A and IFNγ concentration in inguinal lymph node 24 h culture supernatant measured by ELISA. (B) Representative flow cytometry contour plots of encephalitogenic T cells. (C) Quantification of total MNCs, CD3+ T cells, and CD3+CD4+ Th cells per brain. Data presented are mean ± SEM. ^***p < 0.001, ^*p < 0.05 by unpaired two-tailed T-test (A), or Kruskal-Wallis test (C).