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. Author manuscript; available in PMC: 2020 Aug 5.
Published in final edited form as: Curr Biol. 2019 Jul 25;29(15):2547–2554.e2. doi: 10.1016/j.cub.2019.06.055

Figure 4. Nkt is required in mushroom body (MB) α’β’ neurons for normal sleep.

Figure 4.

A) mb247-Gal80 blocks Gal4 activity in the MB but not the ellipsoid body (E) or fan-shaped (FB) body neurons. Signals were enhanced in Photoshop for the right-hand image to document lack of GFP expression in the MB. B) Night sleep phenotype of syb-Gal4 (SG4)>Nkt.IR; mb247-Gal80 (mb-G80) flies and controls. Night sleep for the nSybG4>U-Nkt.IR; mb247G80 population (a 3-day average) is significantly different from that of the nSybG4>U-Nkt.IR knockdown control; p<0.01. C) Flies with 238Y-GAL4-driven knockdown have reduced night sleep and sleep bout fragmentation. This panel shows one of 3 biological replicates with similar results. Knockdown of Nkt with a different pan-MB driver (OK107-GAL4) also reduced night sleep. D, E) Nkt knockdown in the α’β’ lobes of the MB using either R35B12-GAL4 (C, C1) or c305a-GAL4 (D, D1) causes a reduction in night sleep with no effect on day sleep. For C, D and E, histograms represent averages for 3 days of LD data. **, p<0.01; ***, p<0.001 relative to controls.