KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
AAV5-DIO-ChR2(H134R)-eYFP	UNC Vector Core	N/A
Chemicals, Peptides, and Recombinant Proteins
Aprepitant	Selleck Chem	Cat#: S1189
L-733,060 hydrochloride	Tocris	Cat#: 1145
Pirenzepine dihydrochloride	Tocris	Cat#: 1071
SB22220	Tocris	Cat#: 1393
Substance P	Tocris	Cat#: 1156
Guanosine 5′-O-(2-Thiodiphosphate) trilithium salt	Santa Cruz Biotechnology	Cat #: sc-295029
NASPM trihydrochloride	Tocris	Cat #: 2766
BAPTA-tetracesium salt	Thermofisher Scientific	Cat#: B1212
Spermine tetrahydrochloride	Sigma-Aldrich	Cat #: S2876
Critical Commercial Assays
Substance P ELISA	R and D Systems	Cat #: SKGE007
RNAscope multiplex fluorescent manual assay	ACD Bio	Cat #: 320850
Experimental Models: Organisms/Strains
B6;129S-Pdyntm1.1(cre)Mjkr/LowlJ (Dyn-Cre)	Jackson Laboratories	Stock #: 027958
Tg(Adora2a-cre)KG139Gsat/Mmucd (A2a-Cre)	Mutant Mouse Resource and Research Centers (MMRRC)	Stock#: 031168-UCD
B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J (Ai9)	Jackson Laboratories	Stock #: 007909
Oligonucleotides
Mm-Drd1-C1 (nucleotide target region 444–1358)	ACD Bio	GenBank#: NM_010076.3
Mm-Drd2-C2 (nucleotide target region 85–1153)	ACD Bio	GenBank#: NM_010077.2
Mm-Tacr1-C3 (nucleotide target region 845–1775)	ACD Bio	GenBank#: NM_009313.5
Mm-Sst-C1 (nucleotide target region 18–407)	ACD Bio	GenBank#: NM_009215.1
Mm-Chat-C2 (nucleotide target region 1090–1952)	ACD Bio	GenBank#: NM_009891.2
Mm-Chat-C1 (nucleotide target region 1090–1952)	ACD Bio	GenBank#: NM_009891.2
Mm-Ache-C2 (nucleotide target region 316–1318)	ACD Bio	GenBank#: NM_001290010.1
Mm-Chrm1-C1 (nucleotide target region 851–1994)	ACD Bio	GenBank#: NM_001112697.1
Software and Algorithms
pClamp	Molecular Devices	Version 10
Graphpad Prism	Graphpad Software	Version 6 and version 8