Table 1.
Activated FX generation (FXaG) parameters of the the 4-PBA treated rFVII-160R variant and of rFVIIwt, with (+) and without TFPI inhibition
| Anti-TFPI aptamer | rFVII-160R 50 ng/ml |
rFVIIwt 10 ng/ml |
rFVIIwt 5 ng/ml |
|||
|---|---|---|---|---|---|---|
| + | +a | +a | ||||
| Lag time (s) | 348 ± 97 | 261 ± 64 | 205.3 ± 0.1 | 176 | 419.4 ± 9.5 | 354 |
| TTP (s) | 719 ± 96 | 605 ± 51 | 485.0 ± 0.1 | 445 | 778.1 ± 14.4 | 687 |
| Peak (Rfu/s) | 15.0 ± 3.0 | 19.8 ± 2.0 | 18.3 ± 0.3 | 23.0 | 10.8 ± 0.1 | 15.0 |
| AUC (Rfu) | 3127 ± 172 | 3496 ± 111 | 3388 ± 110 | 3694 | 3058 ± 37 | 3401 |
TTP time to peak, RFU relative fluorescence units, AUC area under the curve
aValues reported for rFVIIwt with anti-TFPI addition have been obtained in a single experiment