Fig. 6.

P53-R273H-regulated lncRNAs are implicated in oxaliplatin resistance in colorectal cancer stem cells. a. HCT116 p53 endogenous point mutant (PM) spheroid cells were treated with oxaliplatin (0, 1, 2.5, 5, 10, 20 μM) for 24 h. The relative expression of lnc273–31 and lnc273–34 was assessed by RT-qPCR. b. The IC50 changes after knocking down of lnc273–31 or lnc273–34 in HCT116 p53 endogenous point mutant cells. c. The relative expression of stemness related genes (Sox2, Oct4, Nanog, CD44, ALDH) in HCT116 p53 endogenous point mutant plastic cells (PS), spheroid cells (SS) and oxaliplatin resistant cells (oxa). d. Oxaliplatin-resistant cells in the presence of lnc273–31 or lnc273–34 knock-down were stained with the Aldefluor assay to determine the frequencies of ALDH⁺ cells vs non-treated cells. Experiments were normalized with respective DEAB controls. e. The IC50 changes of knocking down of lnc273–31 or lnc273–34 in oxaliplatin resistant cells. The experiments were performed in triplicate (a-e). Data are shown as means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001