Fig. 6.

Translocation of GalNT2 and GalNT6 towards the trans Golgi after Tamoxifen and Zeocin treatment. a Localization and distribution of GalNT2 and GalNT6 (Alexa 488, green) in the Golgi apparatus of MCF7 cells using the trans-Golgi marker Golgin97 (Alexa 555, red) in comparison to untreated cells. Both cell lines were treated for 48 h by Tam (4 μM) and zeocin (250 μg/ml), respectively. Nuclei colored in blue were stained by DAPI. Scale bar: 20 μm. Magnification of the marked areas are shown. b Mander’s coefficient of the co-localization of GalNTs with the trans Golgi protein Golgin97. Averages and standard deviations of 10 co-localization measurements are given. P-values were calculated with Student’s t-test. **** P < 0.0001. c Western blot analysis of levels of GalNT6 and GalNT2 protein expression in Tam, zeocin and hydrogen peroxide treated MCF7 and T47D cells in comparison to untreated controls (n). Cells were treated as described above. β-Actin served as loading control