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. 2019 Aug 21;7:195. doi: 10.3389/fbioe.2019.00195

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Copyright © 2019 Pappenreiter, Sissolak, Sommeregger and Striedner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Viscosity as a function of shear rate measured in cell suspension and media supplemented with antifoam at 30 and 37°C. (B) Stirrer speed and process air (PA) output as a function of PID controller output (%). (C) Relative dissolved oxygen saturation (DO%) determined in medium upon variation of the molar CO₂ fractions in the inlet gas compared to RO-H₂O. (D) k_La as a function of temperature (T) and PID controller output (%) for water and culture medium supplemented with 0.1% v/v antifoam solution.