Table 1.
Overview of all bioprocess runs at different parameter settings.
| Bioprocess run |
Shift 1 (72 h) |
Shift 2 (120 h) |
Shift 3 (192 h) |
Shift 4 (240 h) |
|---|---|---|---|---|
| 1 | 36.3°C/F3 | – | – | – |
| 2 | 36.3°C/F3 | – | – | – |
| 3 | 34°C/F1 | – | – | – |
| 4 | 37°C/F3 | - | 37°C/F1 | - |
| 5 | 34°C/F2 | 37°C/F2 | 34°C/F1 | 31°C/F1 |
| 6 | 31°C/F2 | 34°C/F2 | 37°C/F3 | 34°C/F3 |
| 7 | 34°C/F1 | 31°C/F1 | 31°C/F2 | 34°C/F2 |
| 8 | 37°C/F2 | 34°C/F3 | 31°C/F2 | 34°C/F1 |
| 9 | 34°C/F3 | 37°C/F2 | 31°C/F2 | 37°C/F3 |
| 10 | 34°C/F2 | – | – | – |
| 11 | 34°C/F2 | – | – | – |
| 12 | 34°C/F2 | – | – | – |
| 13 | 34°C/F2 | – | – | – |
Intra-experimental variations were performed at four shifts in temperature (31, 34, or 37°C), additional D-glucose concentration in the feed medium (+10, +20, or +30 g L−1; identified as F1, F2, F3), or both.