Fig. 3.

Expression of candidate miRNAs in fibrotic tissues. a qRT-PCR validation of top 8 candidate miRNAs in CKD patients. * p < 0.05, **p < 0.01 versus control group (n = 6 in each group). b ISH data using human FFPE sections show that both hsa-miR-4709-3p and has-miR-3607-3p were mainly expressed in tubular epithelial cells. qRT-PCR shows the expression levels of miRNAs in UUO model. Hsa-miR-4709-3p was highly expressed (c) but hsa-miR-3607-3p was decreased (d) in UUO kidney. MCD: minimal change disease; FSGS: focal segmental glomerulosclerosis; DN: diabetic nephropathy; Con: normal kidney tissues from healthy donor. * p < 0.05, **p < 0.01 versus sham group. Data represent means ± SEM for six mice in each group