Fig. 4.

Upregulation of miRNA inhibits the expression level of target genes in HK-2 cells and CKD patients. qRT-PCR shows that transient transfection with hsa-miR-3607-3p (a) and hsa-miR-4709-3p (b) inhibit the mRNA expression of ITGB8 and CALM3 in HK-2 cells respectively. Western Blot shows that hsa-miR-3607-3p (c) and hsa-miR-4709-3p (d) inhibit the protein level of ITGB8 and CALM3 respectively. NC: scrambled negative control transfection; 3607: hsa-miR-3607-3p transfection; 4709: hsa-miR-4709-3p transfection. Western Blot shows that hsa-miR-3607-3p and hsa-miR-4709-3p. Quantitative data of relative protein level of ITGB8 (e) and CALM3 (f) in miRNA mimics transfected cells (normalized to β-actin). Data represent means ± SEM for at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus con; ^#p < 0.05, ^###p < 0.001 versus NC. QRT-PCR of relative mRNA level of ITGB8 (g) and CALM3 (h) in CKD patients (normalized to β-actin). MCD: minimal change disease; FSGS: focal segmental glomerulosclerosis; DN: diabetic nephropathy; Con: normal kidney tissues from healthy donor. * p < 0.05, **p < 0.01 versus control group (n = 6 in each group)