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. 2019 Aug 27;19:840. doi: 10.1186/s12885-019-6029-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Manifestations of PLOD2 maintaining CSC characteristics. a Hep-2 and FaDu cell lines were engineered for the overexpression or silencing of PLOD2. b The SP was analyzed in the different cell lines by multiparametric flow cytometry. c Hep-2 and FaDu cell spheres cultured in medium were photographed; representative images are shown. Scale bar = 50 μm. d The mRNA expression levels of genes representing CSC markers were upregulated. e The protein expression levels of genes representing CSC markers were upregulated. *P < 0.05