Fig. 3.

RX1 and RX2 reduce levels of phospho-STAT3 but only RX2 blocks ICER induction. a Representative Western Blot analysis of whole-cell protein extracts from primary neurons 9 DIV pretreated with100nM Ruxolitinib (RX1), 10uM Ruxo (RX2), or DMSO for 1 h before the addition of BDNF. Cells were collected 30 min after BDNF administration and probed with anti pSTAT3, STAT3 and β-Actin. b Quantitation of signals from densitometry is displayed as mean percent change (±SEM) relative to the DMSO+Water control group (**p < 0.01). Ruxolitinib significantly reduces the levels of P-STAT3 with no change in total STAT3. c Graphical representation of results from real-time PCR analysis using specific Taqman probe and primers. RNA was extracted from cells collected 4 h after BDNF administration. Transcript levels are shown as the mean values (±SEM) of the ratio relative to the DMSO+Water control group for Icer n = 6. Note, that while RX1 blocks pSTAT3, it does not block the effect of BDNF treatment on ICER induction, while WP and RX2 do