FIGURE 9.

The inhibitory effect of CTN on NLRP3 inflammasomes is partially through Nrf2. (A) BV2 microglia cells were treated with CTN at various concentrations for 1 h and subsequently co-treated with 1 μg/ml LPS for 18 h. CTN at concentrations of 5 or 10 μM did not influence the expression of NLRP3, whereas LPS significantly increased the expression of NLRP3, which was attenuated by 10 μM CTN, but not by 5 μM CTN. (B) BV2 microglia cells were treated with CTN at various concentrations for 1 h in the presence or absence of Nrf2 siRNA and subsequently co-treated with 1 μg/ml LPS for 18 h. After transfecting cells with Nrf2 siRNA, the inhibitory effect of 5 μM CTN was partially reversed. Data were presented as mean ± SEM. All experiments were repeated at least three times. ^∗∗P < 0.01, ^∗∗∗P < 0.001.