FIG 3.

PDCD4 silencing by miR-21 and miR-499a-5p in HEK293 (A), UMSCC22B (B), and HeLa (C) cell lines. Cells were cotransfected with 30 pmol of control (i), miR-21 (ii), and miR-499 (iii) mimics in combination with the psi-WT or mutant vectors. Each color indicates a different psi-CHECK vector. The silencing contribution for each miRNA was assessed using relative light units (luciferase activity) and normalized to the value for control cells. Error bars represent SEM (n = 3). *, P < 0.05; **, P < 0.01; ***, P < 0.005 (by one-way ANOVA with a Dunnett test).