TABLE 1.
Common high-score predictions for miR-21 and miR-499 using TargetScana
| Gene transcript (GenBank accession no.) | No. of miR-499-5p conserved predicted sites | No. of miR-499-5p poorly conserved predicted sites | Distance(s) between predicted binding sites for miR-499-5p (nt) | Experimental evidence of interaction |
|
|---|---|---|---|---|---|
| miR-21 | miR-499 | ||||
| SOX6 (NM_001145811) | 5 | 1 | 170, 305, 15, 287, 290 | NA | Luciferase |
| PURB (NM_033224) | 1 | 3 | 1,726, 3,430, 750 | Microarray | NA |
| PDCD4 (NM_001199492) | 3 | 0 | 450, 66 | Luciferase, Western blotting | Luciferase, qRT-PCR, Western blotting |
| JHDM1D (NM_030647) | 1 | 2 | 4,229, 7 | NA | NA |
| SECISBP2L (NM_001193489) | 1 | 2 | 1,080, 1,428 | NA | NA |
| PAIP2B (NM_020459) | 1 | 2 | 729, 52 | NA | NA |
| SOX5 (NM_006940) | 2 | 0 | 158 | Western blotting, Northern blotting, qRT-PCR, luciferase | NA |
| SCRN1 (NM_001145513) | 1 | 1 | 3,334 | Microarray | NA |
| ARHGAP32 (NM_001142685) | 1 | 1 | 49 | NA | NA |
| KLHDC5 (NM_020782) | 1 | 1 | 2,175 | NA | NA |
| RAB22A (NM_020673) | 1 | 1 | 1,650 | NA | NA |
| MEIS1 (NM_002398) | 2 | 0 | 461 | Microarray | NA |
| LRP6 (NM_002336) | 1 | 1 | 576 | NA | NA |
| FOXP2 (NM_001172766) | 1 | 1 | 26 | NA | NA |
| STRN (NM_003162) | 1 | 1 | 1,853 | NA | NA |
a
Each transcript in the table represents at least one conserved binding site predicted for miR-21-5p and at least two binding sites predicted for miR-499a-5p. SOX6, SRY box 6; NA, not applicable.