Figure 2.

Nasal wash and oropharyngeal titres at days 1 and 3 post-challenge. Ferrets were prime-boosted with TIV or viral vectors (ChAdOx1, then MVA) expressing influenza virus antigens (cHA-NP + M1, cHA, or NP + M1). Six weeks after the boost, ferrets were challenged IN with 10⁶ PFU of A/Wyoming/03/2003 (H3N2) virus and nasal washes were collected at days 1 (A) and 3 (B) post-challenge to detect viral titres via plaque assay. Oropharyngeal swabs were also collected at days 1 (C) and 3 (D) post-challenge to determine viral titres. The data are presented as individual replicates and the mean for each group is presented as line. n = 4 ferrets/group. ^**p ≤ 0.01, ^***p ≤ 0.001 when naïve controls were compared to individual vaccination groups as determined by one-way analysis of variance, followed by a Bonferroni multiple-comparison test.