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. 2019 Aug 21;10:2005. doi: 10.3389/fimmu.2019.02005

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Copyright © 2019 McMahon, Asthagiri Arunkumar, Liu, Stadlbauer, Albrecht, Pavot, Aramouni, Lambe, Gilbert and Krammer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antibody responses toward influenza virus antigens in the pre-challenge serum of vaccinated ferrets. Six weeks after the booster vaccination, pre-challenge serum was collected from TIV vaccinated, viral vector vaccinated, and naïve ferrets and IgG antibody responses toward influenza virus antigens were assessed via ELISA. Shown are antibody responses toward the H3 (A), H7 (B), H10 (C), cH4/3 (D), NP (E), and M1 (F) recombinant proteins. HI (G) and microneutralization titres (H) were also assessed. The data are presented as individual replicates and the mean for each group is presented as line. n = 4 ferrets/group. ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p < 0.005 when naïve controls were compared to individual vaccination groups as determined by one-way analysis of variance, followed by a Bonferroni multiple-comparison test.