Figure 6.

IFNγ cellular responses in the MLNs and spleen. TIV vaccinated, viral vector vaccinated (cHA-NP + M1, cHA, or NP + M1), and naïve ferrets were challenged IN with 10⁶ PFU of A/Wyoming/03/2003 (H3N2) virus and at 4 days post-challenge, ferrets were euthanized, and spleens and MLNs were removed to enumerate influenza virus-specific cellular responses. Splenocytes and MLN cells were stimulated with peptide arrays spanning the NP + M1 fusion protein and influenza virus-specific IFN-γ expressing cells were detected in the MLNs (A) and spleens (B) by ELISpot. The ICS assay was also used to detect influenza virus-specific IFNγ⁺CD8⁺ T cells in the MLN (C) and spleen (D). The data are presented as individual replicates and the mean for each group is presented as line. n = 4 ferrets/group. ^**p ≤ 0.01, ^***p ≤ 0.001 when naïve controls were compared to individual vaccination groups as determined by one-way analysis of variance, followed by a Bonferroni multiple-comparison test.