Figure 2.

α‐dystroglycan surface expression and virus binding is reduced on LNCaP. (a) Surface expression of α‐dystroglycan was analyzed by flow cytometry using specific antibodies or only secondary antibody as control. (b) Binding capacity of VSV‐GP to prostate cancer cells was analyzed. Cells were prepared to a single‐cell suspension using nonenzymatic methods and infected with VSV‐GP at 4°C or treated with PBS for control. After 30 min, cells were washed several times, fixed, stained with antibodies specific for GP and analyzed by flow cytometry.