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. 2019 Aug 21;9:776. doi: 10.3389/fonc.2019.00776

Figure 4.

Figure 4

Inhibition of LINC00957 reversed 5-FU resistance by regulating P-gp expression. (A) In LOVO/5-FU and HCT8/5-FU cell lines, LINC00957 expression was examined by qRT-PCR in the situations of non-transfected, si-control, siRNA1, siRNA2 and siRNA3. #stood for the best silent effect, **stood for P < 0.001. (B) Inhibition of LINC00957 by siRNA2 reversed 5-FU drug resistance in LOVO/5-FU cell lines. (C) Inhibition of LINC00957 by siRNA2 reversed 5-FU drug resistance in HCT/5-FU cell lines. (D) The relative multi-drug resistance genes(ABCB1, ABCG2, ABCC1, ABCC2) were examined in LOVO/5-FU and LOVO/5-FU/siRNA2,also in HCT8/5-FU and HCT8/5-FU/si-RNA2. The ABCB1 was significantly down-regulated by inhibition of LINC00957 in LOVO/5-FU and HCT8/5-FU. *stood for P < 0.05. (E) The expression of P-gp, which is coded by ABCB1,was detected by western blot. (F) The expression of P-gp was significantly down-regulated by inhibiting LINC00957 (siRNA2) than normal as control (NC) in LOVO/5-FU and HCT8/5-FU cell lines, respectively.