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. 2019 Aug 21;7:171. doi: 10.3389/fcell.2019.00171

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Copyright © 2019 Saraste and Prydz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IC elements and REs persist and co-localize during mitosis. Normal rat kidney (NRK) cells stably expressing green fluorescent protein (GFP)-coupled Rab1 as a marker for the IC were labeled with fluorescent transferrin during a 1 h uptake to visualize the endosomal recycling system. At the same time, the cells were exposed to BFA, which disassembles the Golgi stacks, but does not affect mitotic entry or progression. Note the co-localization of the IC elements and REs at the spindle poles of a cell that has reached prometaphase (open arrows), as well as in the pericentrosomal area of interphase cells (arrows) and. In addition, co-localization of the two markers is observed at peripheral sites (arrowheads). The interphase nuclei and mitotic chromosomes are stained with DAPI. Bar = 5 μm (see also Marie et al., 2012; Takatsu et al., 2013).