Figure 1.

(A) LDW setup. Scheme of the laser-based direct-write setup, which shows the deposition head printing the liquid photopolymer onto the nitrocellulose membrane and its subsequent photo-polymerization by the laser beam. (B) Duplex LFD specific for human VL (VL-LFD). Schematic diagram of the proposed new LDW LFD with double-channel geometry as a rapid serodiagnostic assay for human VL. The assay is based on a laser-patterned microfluidic device on a low-cost paper platform, using recombinant β-tubulin and LiHyp1 antigens.