(A) For our discovery set, mammary cell preparations (MCPs) were isolated from caudal glands of 12-week-old NRL-PRL females (MCPs A–E, n = 5). Nulliparous mice with matched end-stage tumors (T) and adjacent aged mammary glands (AMGs) were collected following development of spontaneous ER+ tumors (15–20 months; T1–T5, AMG1–AMG5, n = 5). All samples were examined by RNA-seq. Tumors and matched tail DNA samples underwent WGS and WES.
(B) In our extension set, we evaluated 22 additional tumors from NRL-PRL females (T6–T27) and 4 cell lines (derived from 2 independent tumors).
(C) Summary of samples interrogated in this study. The number of mice associated with each group or the sample identifiers are indicated in parentheses. *Four cell lines were derived from 2 additional independent tumors (2 each).
See Table S1 for further details.