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. Author manuscript; available in PMC: 2019 Aug 28.
Published in final edited form as: Cell Rep. 2019 Aug 6;28(6):1526–1537.e4. doi: 10.1016/j.celrep.2019.06.098

Figure 6. Validation of RNA-Seq Findings.

Figure 6.

(A–F) RT-PCR was used to evaluate transcripts associated with cellular processes altered with oncogenesis (identified in Figure 5) across samples in the discovery set (MCP, AMG, and tumor; indicated by †) as well as aged mammary cell preparations from NRL-PRL females (AMCP). These include fatty acid metabolism (A), hormone receptors (B), signaling pathways (C), cell cycle (D), cell adhesion (E), and immune processes (F). MCPs were also isolated from wild-type young adults (WT MCP) (n = 4–5). Data are represented as mean ± SEM. Significant differences were determined by one-way ANOVA, followed by the Tukey post-test. *p < 0.05, **p < 0.01, and ***p < 0.001.

(G) Immunohistochemistry demonstrating higher levels of CCND1 expression in tumors compared with preneoplastic tissue (i), low levels of CD8+ intratumoral lymphocytes compared with mammary lymphoid tissue (inset, ii), and F4/80+ myeloid cells within tumors and stroma and surrounding preneoplastic structures (inset, iii). Scale bars, 50 μm.