Figure 4.

Highly-Enriched Proteins in SCD11 and SCD14 Coimmunoprecipitates.

(A) Proteins whose average (Avg) enrichment from lines expressing SCD11 or SCD14 in comparison to the Col-0 progenitor was three or greater. Stars mark two RNA binding proteins of unknown function. HCF173 (hashtag) is known to associate with psbA mRNA and to activate its translation (Schult et al., 2007; Williams-Carrier et al., 2019). The full data set is provided in Supplemental Data Set 2, which includes a more complete explanation of the data analysis.

(B) Immunoblot validation of two proteins identified by MS analysis. Chloroplast stroma from plants expressing SCD11 or SCD14, or from the Col-0 progenitor was used for immunoprecipitation with anti-FLAG antibody. Replicate immunoblots were probed with anti-FLAG antibody to detect SCD11 and SCD14 (top), HCF173 (middle), or cpSRP54 (bottom). The HCF173 blot was initially probed to detect RbcL (the large subunit of Rubisco), an abundant protein that typically contaminates immunoprecipitates; this serves as an internal standard.