Table 4. Selectable Promoters in Use in Chlamydomonas Transformation.
Promoter | Expression Type | Origin | Reference |
---|---|---|---|
RBCS2 | Constitutive | Chlamydomonas | (Stevens et al., 1996) |
PsaD | Constitutive | Chlamydomonas | (Fischer and Rochaix, 2001) |
HSP70A | Constitutive, heat-inducible | Chlamydomonas | (Schroda et al., 2000) |
RBCS2:HSP70A | Constitutive, heat-inducible | Chlamydomonas | (Schroda et al., 2000) |
NIT1 | Ammonium-inducible, nitrogen-repressible | Chlamydomonas | (Ohresser et al., 1997) |
CYC6 | Nickel-inducible, copper-repressible | Chlamydomonas | (Pérez-Martín et al., 2015) |
THI4a | Thiamine-repressible | Chlamydomonas | (Croft et al., 2007) |
METE | Vitamin B12-repressible | Chlamydomonas | (Helliwell et al., 2014) |
35S | Constitutive | CaMV | (Díaz-Santos et al., 2013) |
Nopaline synthase (NOS) | Constitutive | Agrobacterium | (Díaz-Santos et al., 2013) |
THI4 is a riboswitch found in the THI4 transcript that provides thiamine-repressible control of gene expression when fused to a promoter.