Figure 2.

Identification and Molecular Characterization of the EOD9 Gene.

(A) MED16/EOD9 gene structure, showing the mutation site of eod9-1 and the T-DNA insertion site of med16-2. Black boxes represent exons, lines represent introns, and the white box represents the 3′ untranslated region.

(B) and (C) Plants before bolting (B) and the sixth leaves (C) of Col-0, eod9-1, med16-2, gMED16;eod9-1 #1 (COM#1), and gMED16;eod9-1 #2 (COM#2) (from left to right). The gMED16;eod9-1 plants were generated by transforming eod9-1 plants with a genomic fragment (gMED16) containing the 2080-bp promoter and the MED16 (At4g04920) gene.

(D) and (E) Petals (D) and flowers (E) of Col-0, eod9-1, med16-2, COM#1, and COM#2 plants (from left to right).

(F) Leaf area (LA), leaf length (LL), and leaf width (LW) of the sixth leaves of Col-0, eod9-1, med16-2, COM#1, and COM#2 plants (n = 12).

(G) Petal area (PA), petal length (PL), and petal width (PW) of Col-0, eod9-1, med16-2, COM#1, and COM#2 plants (n = 80).

(H) and (I) Inflorescences (H) and number of flower buds (I) of Col-0, eod9-1, med16-2, COM#1, and COM#2 plants (n = 6).

(J) to (O) The expression patterns of MED16 in 9-d-old seedlings (J), developing petals (K), stamens (L), sepals (M), siliques (N), and root tips (O) of MED16pro:GUS plants.

(P) The expression levels of MED16 in the first leaf (1 th), second leaf (2 th), and third leaf (3 th) from 9-d-old Col-0 seedlings and 10th stage petals (10 st), 12th stage petals (12 st), and 14th stage petals (14 st) from Col-0 flowers (n = 3).

Error bars represent se. Asterisks indicate significant differences from Col-0: * P < 0.05 and ** P < 0.01. Bars = 4 cm (B), 1 cm (C), 0.25 cm ([J] and [N]), 3 mm (H), 1 mm ([D], [E], [K], and [M]), 0.5 mm (L), and 100 μm (O).