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. 2019 May 24;31(8):1788–1806. doi: 10.1105/tpc.18.00918

Figure 1.

Figure 1.

TINY Negatively Regulates Plant Growth by Inhibiting the BR Pathway.

(A) Growth phenotype of the 4-week-old wild-type (WT) and TINY-OE (lines 3 and 8) plants. The sixth leaves of plants were measured for petiole length. Data represent mean and sd, n = 30 to 36. *P < 0.05, **P < 0.01; Student’s t test.

(B) Phenotype of the 4-week-old wild-type (WT) and tiny tiny2 tiny3 plants. Data represent mean and SD, n = 15 to 22.

(C) and (D) BRZ sensitivity of TINY-OE and tiny tiny2 tiny3 plants. Seven-day-old seedlings were grown on 1/2 LS medium with 250 nM BRZ in dark (C). Hypocotyl was measured using ImageJ (D). Data represent mean and sd from three biological replicates (n = 3); each replicate contained 12 to 15 seedlings. *P < 0.05, **P < 0.01; two-way analysis of variance. WT, wild type.

(E) and (F) BR-regulated gene expression in TINY mutants. mRNA was extracted from 4-week-old plants for BR-induced genes (E) and BR-repressed genes (F) expression analysis. Data represent mean and sd from three biological replicates (n = 3). Each biological replicate was pooled tissue from three to four individual plants. *P < 0.05, **P < 0.01; Student’s t test.