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. 2019 Aug 28;15(8):e1007964. doi: 10.1371/journal.ppat.1007964

Fig 1. Domain organization and expression profile of the seven chitin synthases in M. acridum.

Fig 1

(A) Domain structures of the seven chitin synthases in M. acridum. Chs, chitin synthase domain; MMD, myosin motor domain; Cyt-b5, cytochrome b5-like heme/steroid binding domain; TM, transmembrane domain. (B) The expression levels of seven Chs genes in conidia. Conidia harvested from 15-day-old 1/4 SDAY cultures. (C) The expression levels of seven Chs genes in hyphae. Hyphae harvested from 18-hour-old 1/4 SDAY cultures. (D) The expression levels of seven Chs genes during germination on the locust hind wings. Samples were collected at 8 h after inoculation on the locust hind wings. (E) The expression levels of seven Chs genes during appressorium formation on the locust hind wings. Samples were collected at 20 h after inoculating on the locust hind wings. (F) The expression levels of seven Chs genes in hyphal bodies. Hyphal bodies were harvested from locust hemolymph at 5 d after inoculation. Expression profiles of seven MaChs genes assayed by qRT-PCR. The expression level of MaChsI was arbitrarily set to 1. Standard errors were determined with data from three independent replicates. Different lowercase letters on bars denote significant difference, P < 0.05.