(A) The hydrophobicity of conidia from wild type, ΔMaChsIII, ΔMaChsV, ΔMaChsVII mutants. (B) Germination time values for 50% germination (GT50) of wild type, ΔMaChsIII, ΔMaChsV, ΔMaChsVII mutants on locust hind wings. (C) Rates of appressorium formation in germinated conidia. (D) Appressorium morphology of wild type, ΔMaChsIII, ΔMaChsV, ΔMaChsVII mutants. White arrows represent the various defects of conidia and appressoria. (E) LC50 of PEG-8000 of collapsed appressoria. (F) The relative expression levels of Pr1A gene (GenBank accession number: AAV97788) in mature conidia and appressoria (after induced 30 h on locust wings) of wild type, ΔMaChsIII, ΔMaChsV, ΔMaChsVII mutants. A single asterisk above bars denotes significant difference, P < 0.05; double asterisks above bars denote significant difference, P < 0.01. Error bars indicate standard errors of three trials.