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. 2019 Aug 28;14(8):e0221798. doi: 10.1371/journal.pone.0221798

Fig 4. Immunohistochemical stain for two isoforms of MMP-2.

Fig 4

(A) IHC expression of the two MMP-2 isoforms in transverse sectional images (papillary muscle level) of control and STZ-induced diabetic mouse hearts. MMP-2 expressing areas were colored using yellow or red dots using a staining intensity algorithm. a: FL-MMP-2 staining was minimal at 12 weeks after streptozotocin injection control mice. b: NTT-MMP-2 was not expressed in non-diabetic control mice. c: FL-MMP2 was expressed strongly in the near whole area of the section in diabetic mouse hearts. d: NTT-MMP-2 was also strongly expressed and its expression pattern was similar to that of FL-MMP-2 in diabetic mouse hearts. (B) Stained pixel numbers were counted using the Aperio Positive Pixel Count Algorithm. IHC pixel density analysis of FL- and NTT-MMP-2 in transverse section images showed greater expressions of both MMP-2 isoforms in diabetic mouse heart compared to the control group (p<0.05) (by Aperio Image Scope v12.3.2.8013, Leica Biosystems Pathology Imaging, USG).