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. 2019 Aug 28;14(8):e0220694. doi: 10.1371/journal.pone.0220694

Fig 1. Plasmid architecture in the COSPLAY toolbox.

Fig 1

A) Schematic representation of an expression vector assembled by COSPLAY composed of 6 independent modules. Each module represents a different type of sequence as indicated on the legend. B) Restriction site of the BsaI endonuclease and specific overhangs used; C) Principle of COSPLAY expression vector assembly. A destination vector (pDV) is mixed with 6 plasmids containing compatible module in a single tube. Expression vector is assembled through simultaneous digestion/ligation in the presence of BsaI and T4 DNA ligase. Specific overhangs that ensure that individual modules are assembled in the right order and orientation are indicated in pink.