Table 3. COSPLAY library of modules.
| ID | Position | Type | Description | Backbone | Resistance |
|---|---|---|---|---|---|
| pMV14 | 1 | plasmid type | 2μ | pUC57 | AmpR |
| pMV8 | 1 | plasmid type | linker (Yip) | pUC57 | AmpR |
| pMV13 | 1 | plasmid type | CEN | pUC57 | AmpR |
| pMV462 | 1 | plasmid type | VI260998 (Yip) | pUC57 | AmpR |
| pMV473 | 1 | plasmid type | IX301997 (Yip) | pUC57 | AmpR |
| pMV1 | 2 | Promoter | GAL1P | pUC57 | AmpR |
| pMV2 | 2 | Promoter | MET25p | pUC57 | AmpR |
| pMV17 | 2 | Promoter | ACT1p | pUC57 | AmpR |
| pMV19 | 2 | Promoter | ADH1p | pUC57 | AmpR |
| pMV20 | 2 | Promoter | CYC1p | pUC57 | AmpR |
| pMV21 | 2 | Promoter | TEF1p | pUC57 | AmpR |
| pMV48 | 2 | Promoter | TDH3p | pUC57 | AmpR |
| pMV31 | 3 | Reporter gene | sfGFP | pUC57 | AmpR |
| pMV41 | 3 | Reporter gene | mCherry | pUC57 | AmpR |
| pMV221 | 3 | Reporter gene | Venus | pUC57 | AmpR |
| pMV451 | 3 | NLS sequence | 3SV40NLS | pUC57 | AmpR |
| pMV5 | 4 | Cln2-PEST Degron | CLN2-PEST | pUC57 | AmpR |
| pMV23 | 4 | STOP codon | STOP | pUC57 | AmpR |
| pMV42 | 4 | Reporter gene | mCherry-STOP | pUC57 | AmpR |
| pMV44 | 4 | Reporter gene | sfGFP-STOP | pUC57 | AmpR |
| pMV6 | 5 | Terminator | ADH1t | pUC57 | AmpR |
| pMV7 | 6 | Selection marker | TRP1 | pUC57 | AmpR |
| pMV10 | 6 | Selection marker | URA3 | pUC57 | AmpR |
| pMV12 | 6 | Selection marker | NatMX | pUC57 | AmpR |
| pMV16 | 6 | Selection marker | LEU2 | pUC57 | AmpR |
| pMV18 | 6 | Selection marker | KanMX | pUC57 | AmpR |
| pDV | N/A | Destination Vector | pUC57 with 2 BsaI sites flanking a LacZ cassette and carrying an SV40 polyA signal | pUC57 | CmR |
| pUC57 | N/A | Module Cloning Vector | pUC57 with no BsaI sites and carrying a LacZ cassette | pUC57 | AmpR |
1 pMV3, pMV4, pMV22, pMV45 modules do not have a STOP codon (fused to module 4)
2 pMV46 corresponds to a target locus situated in chromosome VI (position 260998 to 261148)
3 pMV47 corresponds to a target locus situated in chromosome IX (position 301997 to 302147)