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. 2019 Aug 13;44(4):1309–1324. doi: 10.3892/ijmm.2019.4311

Figure 5.

Figure 5

H2 alleviates mitochondrial dysfunction in macrophages induced by LPS via autophagy-mediated NLRP3 inactivation. Macrophages were treated with LPS, ATP and H2, autophagy inducer Rap and autophagy inhibitor 3-MA. Cells were harvested to measure (A) MMP, (B) RCR, (C) ATP, (D) mtDNA and (E) ROS release. Data are expressed as the mean ± standard deviation (n=6). *P<0.05 vs. LPS group, **P<0.05 vs. LPS+H2 group, ##P<0.05 vs. LPS+H2+NLRP3 inhibitor group. LPS, lipopolysaccharide; Rap, rapamycin; 3-MA, 3-methyladenine; MMP, mitochondrial membrane potential; RCR, respiratory control ratio; mtDNA, mitochondrial DNA; ROS, reactive oxygen species; H2, hydrogen.