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. 2019 Aug 13;44(4):1309–1324. doi: 10.3892/ijmm.2019.4311

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of autophagy-mediated NACHT, LRR and PYD domains-containing protein 3 inactivation on lung injury, biochemical parameters of liver and kidney and survival rate in LPS-induced macrophages treated with H₂. Sepsis was produced by CLP. Septic mice were treated with H₂, autophagy inducer Rap and autophagy inhibitor 3-MA. After 24 h, lung tissues were collected to detect (A) pathological tissue changes by hematoxylin and eosin staining, (B) MPO activity and (C) W/D weight ratio; (D) bronchoalveolar lavage fluid was collected to analyze total proteins. (E) Liver and (F) kidney tissues were collected to investigate pathological scores. Blood was obtained to measure the biochemical parameters (G) ALT, (H) AST, (I) Cr and (J) BUN. (K) Survival rate was analyzed at 1, 2, 3, 5 and 7 days post-CLP (n=20). Data are expressed as the mean ± standard deviation (n=6). ^*P<0.05 vs. Con group, ^**P<0.05 vs. CLP group, ^#P<0.05 vs. CLP+H₂ group. LPS, lipopolysaccharide; CLP, cecal ligation and puncture; Rap, rapamycin; 3-MA, 3-methyladenine; MPO, myeloperoxidase, W/D, wet/dry; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; Cr, creatinine; H₂, hydrogen; Con, control.

Effect of autophagy-mediated NACHT, LRR and PYD domains-containing protein 3 inactivation on lung injury, biochemical parameters of liver and kidney and survival rate in LPS-induced macrophages treated with H₂. Sepsis was produced by CLP. Septic mice were treated with H₂, autophagy inducer Rap and autophagy inhibitor 3-MA. After 24 h, lung tissues were collected to detect (A) pathological tissue changes by hematoxylin and eosin staining, (B) MPO activity and (C) W/D weight ratio; (D) bronchoalveolar lavage fluid was collected to analyze total proteins. (E) Liver and (F) kidney tissues were collected to investigate pathological scores. Blood was obtained to measure the biochemical parameters (G) ALT, (H) AST, (I) Cr and (J) BUN. (K) Survival rate was analyzed at 1, 2, 3, 5 and 7 days post-CLP (n=20). Data are expressed as the mean ± standard deviation (n=6). ^*P<0.05 vs. Con group, ^**P<0.05 vs. CLP group, ^#P<0.05 vs. CLP+H₂ group. LPS, lipopolysaccharide; CLP, cecal ligation and puncture; Rap, rapamycin; 3-MA, 3-methyladenine; MPO, myeloperoxidase, W/D, wet/dry; ALT, alanine transaminase; AST, aspartate transaminase; BUN, blood urea nitrogen; Cr, creatinine; H₂, hydrogen; Con, control.