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. 2019 Aug 13;44(4):1309–1324. doi: 10.3892/ijmm.2019.4311

Figure 9.

Figure 9

H2 alleviates cytokine release and NLRP3 inflammasome activation in the kidneys of septic mice via increasing autophagy. Septic mice were treated with H2, autophagy inducer Rap and autophagy inhibitor 3-MA. After 24 h, kidney tissues were collected to detect cytokines (A) IL-1β, (B) IL-18 and (C) TNF-α by ELISA and the expression of (D) caspase-1, (E) NLRP3 and (F) ASC by western blotting. Data are expressed as the mean ± standard deviation (n=6). *P<0.05 vs. Con group, **P<0.05 vs. CLP group, #P<0.05 vs. CLP+H2 group. Rap, rapamycin; 3-MA, 3-methyladenine; IL, interleukin; TNF, tumor necrosis factor; NLRP3, NACHT, LRR and PYD domains-containing protein 3; ASC, apoptosis-associated speck-like protein containing a CARD; CLP, cecal ligation and puncture; H2, hydrogen; Con, control.