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. 2019 Jul 24;44(4):1219–1232. doi: 10.3892/ijmm.2019.4287

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Copyright: © Gong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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miR-29a promotes cell apoptosis of U2OS and MG-63 osteosarcoma cells through the SOCS1/NF-κB signalling pathway. (A) mRNA expression of SOCS1 in cells transfected with miR-29a mimics and/or siSOCS1, assessed by reverse transcription-quantitative polymerase chain reaction. (B) Western blots and (C) quantified protein level of SOCS1 in cells transfected with miR-29a mimics and/or siSOCS1. (D) Flow cytometry results and (E) quantified apoptosis rate of cells transfected with miR-29a mimics and/or siSOCS1. (F) Western blots and (G) quantified protein levels of cleaved caspase-3, uncleaved caspase-3, cleaved PARP, uncleaved PARP, Bax and Bcl-2 in cells transfected with miR-29a mimics and/or siSOCS1. Data are expressed as the mean ± standard deviation. Comparison among three or more groups was conducted using one-way analysis of variance followed by Tukey's post hoc test. ^*P<0.05, ^**P<0.01 and ^***P<0.001. miR-29a, microRNA-29a; SOCS1, suppressor of cytokine signalling 1; siSOCS1, SOCS1 siRNA; NC, negative control; PARP, poly (ADP-ribose) polymerase; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein.

miR-29a promotes cell apoptosis of U2OS and MG-63 osteosarcoma cells through the SOCS1/NF-κB signalling pathway. (A) mRNA expression of SOCS1 in cells transfected with miR-29a mimics and/or siSOCS1, assessed by reverse transcription-quantitative polymerase chain reaction. (B) Western blots and (C) quantified protein level of SOCS1 in cells transfected with miR-29a mimics and/or siSOCS1. (D) Flow cytometry results and (E) quantified apoptosis rate of cells transfected with miR-29a mimics and/or siSOCS1. (F) Western blots and (G) quantified protein levels of cleaved caspase-3, uncleaved caspase-3, cleaved PARP, uncleaved PARP, Bax and Bcl-2 in cells transfected with miR-29a mimics and/or siSOCS1. Data are expressed as the mean ± standard deviation. Comparison among three or more groups was conducted using one-way analysis of variance followed by Tukey's post hoc test. ^*P<0.05, ^**P<0.01 and ^***P<0.001. miR-29a, microRNA-29a; SOCS1, suppressor of cytokine signalling 1; siSOCS1, SOCS1 siRNA; NC, negative control; PARP, poly (ADP-ribose) polymerase; Bcl-2, B-cell lymphoma-2; Bax, Bcl-2-associated X protein.