miR-29a and SOCS1 are downregulated, and DNMT3B is upregulated in OS cells. (A) Expression levels of miR-29a, DNMT3B and SOCS1 in different OS cell lines and normal hFOB1.19 cells, assessed by reverse transcription-quantitative PCR. (B) Methylation level of SOCS1 in different OS cell lines and normal hFOB1.19 cells, examined by methylation-specific PCR. (C) Western blots and (D) quantified protein levels of DNMT3B and SOCS1 in three OS cell lines and normal hFOB1.19 cells. (E) Representative wound healing assay images (magnification, ×40) and (F) migration distance of different OS cell lines. Data are expressed as the mean ± standard deviation. Comparison among three or more groups was conducted using one-way analysis of variance followed by Tukey's post hoc test. *P<0.05, **P<0.01 and ***P<0.001. miR-29a, microRNA-29a; SOCS1, suppressor of cytokine signalling 1; DNMT3B, DNA methyltransferase 3B; OS, osteosarcoma; PCR, polymerase chain reaction; UM con, unmethylated control; M con, methylated control.