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. 2019 Aug 28;5(8):eaax0801. doi: 10.1126/sciadv.aax0801

Fig. 1. Tethering siRNA to the hydrogel via Michael-addition chemistry and its release.

Fig. 1

Schematic illustration of (A) siRNA conjugation to DEX-MAES and (B) hydrogel formation via photocrosslinking and siRNA release upon hydrolytic degradation. (C) Acrylamide gel showing conjugation of siRNA-SH to DEX-MAES over time. (D) Release profiles of unbound (physically trapped siGFP, “unbound siGFP”) and covalently tethered siGFP-SH (“bound siGFP-SH”) from 10% (w/w) DEX hydrogels (20 μg of RNA per 50 μl of gel) (*P < 0.001 compared with “unbound siGFP” at the same time point). UV, ultraviolet; PBS, phosphate-buffered saline; Ds siRNA, double-stranded siRNA; bp, base pairs.